Chapter 6: Growth and Culturing of Bacteria

I. Growth
     A. Bacteria are unicellular
     B. Don't grow in size (except to divide)
     C. Growth means replication
     D. How do nutrients get into the cell?
II. Growth requirements
     A. Physical
     B. Chemical
III. Temperature
     A. Range
     B. Psychrophiles
     C. Psychrotrophs
     D. Mesophiles
     E. Thermophiles
     F. Hyperthermophiles
     G. Temperature and food storage
     H. Temperature and disease
IV. pH
     A. Measure of acidity or alkalinity
     B. Acidophiles
     C. Buffers
V. Osmotic pressure (Water availability)
     A. Definition
     B. Hypertonic
     C. Hypotonic
     D. Osmotolerant
     E. Halophiles
     F. Practical applications
VI. Chemical requirements for growth
     A. Carbon source
     B. Nitrogen source
     C. Hydrogen
     D. Sulfur
     E. Oxygen
     F. Phosphorous
     G. Trace elements
     H. Growth factors
     I. Also need energy sources
VII. Different microbes have different oxygen requirements
     A.  Catabolism produces toxic oxygen compounds
     B. Some bacteria have enzymes which can detoxify these compounds
VIII. Classification based on oxygen requirements
     A. Aerobes
     B. Anaerobes
IX. Organic growth factors
     A. Essential organic compounds that organisms cannot synthesize
     B. Vary from species to species
X. Growing bacteria in the lab
     A. Culture (growth) media
     B. Forms of media
     C. Types of media
XI. Media for special growth requirements
     A. Anaerobic growth media and methods
     B. Special culture techniques
XII. Media used in identifying bacteria
     A. Selective media
     B. Differential media
     C. Media that are both selective and differential
     D. Enrichment media
     E. Special media
XIII. Laboratory techniques
     A. Obtaining pure cultures
     B. Long-term storage of bacteria
XIV. Bacterial growth
     A. Not in size, but in numbers
     B. Generation time
     C. Bacterial growth curve
XV. Measuring bacterial growth
     A. Cell number
     B. Cell mass
     C. Cell concentration (turbidity) [note: book considers this to be biomass]
     D. Metabolic activity
 
 

For questions, comments and additional information, contact  mfhicks@pstcc.edu
Last Updated: January 15, 2004
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